Effects of purposeful soccer heading on circulating small extracellular vesicle concentration and cargo     

Eric R. Muoz  Jaclyn B. Caccese  Brittany E. Wilson  Kyle T. Shuler  Fernando V. Santos  Carolina T. Cabn  John J. Jeka  Dianne Langford  Matthew B. Hudson 《运动与健康科学(英文)》2021,10(2):122

BackgroundConsidering the potential cumulative effects of repetitive head impact (HI) exposure, we need sensitive biomarkers to track short- and long-term effects. Circulating small extracellular vesicles (sEVs) (<200 nm) traffic biological molecules throughout the body and may have diagnostic value as biomarkers for disease. The purpose of this study was to identify the microRNA (miRNA) profile in circulating sEVs derived from human plasma following repetitive HI exposure.MethodsHealthy adult (aged 18–35 years) soccer players were randomly assigned to one of 3 groups: the HI group performed 10 standing headers, the leg impact group performed 10 soccer ball trapping maneuvers over 10 min, and the control group did not participate in any soccer drills. Plasma was collected before testing and 24 h afterward, and sEVs were isolated and characterized via nanoparticle tracking analysis. Next-generation sequencing was utilized to identify candidate miRNAs isolated from sEVs, and candidate microRNAs were analyzed via quantitative polymerase chain reaction. In silico target prediction was performed using TargetScan (Version 7.0; targetscan.org) and miRWalk (http://mirwalk.umm.uni-heidelberg.de/) programs, and target validation was performed using luciferase reporter vectors with a miR-7844-5p mimic in human embryonic kidney (HEK) 293T/17 cells.ResultsPlasma sEV concentration and size were not affected across time and group following repetitive HI exposure. After 24 h, the HI read count from next-generation sequencing showed a 4-fold or greater increase in miR-92b-5p, miR-423-5p, and miR-24-3p and a 3-fold or greater decrease in miR-7844-5p, miR-144-5p, miR-221-5p, and miR-22-3p. Analysis of quantitative polymerase chain reaction revealed that leg impact did not alter the candidate miRNA levels. To our knowledge, miR-7844-5p is a previously unknown miRNA. We identified 8 miR-7844-5p mRNA targets: protein phosphatase 1 regulatory inhibitor subunit 1B (PPP1R1B), LIM and senescent cell antigen-like domains 1 (LIMS1), autophagy-related 12 (ATG12), microtubule-associated protein 1 light chain 3 beta (MAP1LC3B), integrin subunit alpha-1 (ITGA1), mitogen-activated protein kinase 1 (MAPK1), glycogen synthase kinase 3β (GSK3β), and mitogen-activated protein kinase 8 (MAPK8).ConclusionCollectively, these data indicate repetitive HI exposure alters plasma sEV miRNA content, but not sEV size or number. Furthermore, for the first time we demonstrate that previously unknown miR-7844-5p targets mRNAs known to be involved in mitochondrial apoptosis, autophagy regulation, mood disorders, and neurodegenerative disease.  相似文献   

2018年U18女篮亚青赛日本队进攻特点研究     

高猛  徐校飞  龚闯 《体育科技文献通报》2020,(1):58-61,73

凭借着顽强的团队作风和快速灵活的打法,日本女篮近些年多次站在亚洲最高领奖台上,成为中国队的劲敌,无论是青年队还是成年队都给中国队前进路上带来了不少阻碍。俗话说“知己知彼,百战不殆”。本研究运用录像视频分析法、文献资料法及数理统计法,对获得2018年U18女篮亚青赛亚军的日本队进攻特点进行研究与分析。通过对日本队的进攻特点进行研究与分析从而为我国青少年篮球后备人才的培养提供更加广阔的参考依据与培养方案。  相似文献   

An exploration of young professional football players’ perceptions of the talent development process in England     

《Sport Management Review》2020,23(3):536-547

The identification and development of players in English professional football has become an increasingly significant topic of debate given the historical perceived underperformance of the English national team at international tournaments. To enhance understanding of the challenges and barriers experienced by English youth footballers, the authors explore the developmental experiences of English professional football players from different levels of the English football pyramid. Professional players (N = 11) from football clubs in the top four professional divisions in England took part in individual semi-structured interviews, which were analysed inductively using thematic analysis. The data revealed three interrelated themes that were perceived to mediate player identification and development pathways at professional clubs. Pathways for young players to progress and experience first-team competitive football differed when the level of the league that the players operated within was considered, with significant issues also raised relating to the suitability of the under 21 league structure, the importance attached to the educational welfare of young players, and variations in the identification of player attributes. This study sheds new light on the priorities and processes of talent development and education provision in English football.  相似文献   

教育科学化的早期探索——欧美教育科学研究运动简析     

郭法奇 《教育文化论坛》2021,(1):1-8

19世纪后期至20世纪初期,欧美出现的教育科学研究运动是教育研究领域一次具有深远意义的运动它的出现与这个时期欧美社会新的发展和教育的深刻变革,以及人们对科学、科学与教育关系的认识有密切的联系。根据欧美教育科学研究运动中一些突出的事件加以划分,其发展可以分为四个阶段,且每个阶段都有其显著的特点。19世纪末至20世纪初期,欧美出现的教育科学研究运动是现代科学研究思想和方法在教育研究领域里的初次运用和实践,其所关注的领域和所争论的问题值得今天反思与借鉴。  相似文献   

日记风波与《申报》纠纷--郭嵩焘毁誉的西方媒介因素论析     

于翠玲 《北京师范大学学报(社会科学版)》2006,(2):76-81

郭嵩焘在出使英国期间,因《使西纪程》而遭到非议;又因《申报》的报道引起名誉纠纷。这两次事件反映了中西传播环境的差异,以及西方新闻媒介对中国官方舆论的微妙影响。郭嵩焘日记有关《泰晤士报》等西方媒介的介绍,具有重要的新闻史料价值,对中国戊戌变法前后出现的办报高潮也有铺垫作用。  相似文献   

Access to Public-Sector Information in Europe: Policy, Rights, and Obligations     

Michael Blakemore  Max Craglia 《The Information Society》2006,22(1):13-24

The article reviews the debates and policies on access to public-sector information (PSI) in Europe in relation to the contests between policies of open access, rights of access to PSI by citizens and business, and the assessment of the cost benefits of PSI to the economy and society. The political dimension of these debates within the European Union is highlighted to demonstrate the complexities of the governance of information within a pan-European regulatory framework.  相似文献   

National Information Infrastructures in Central and Eastern Europe: Perspectives from the Library Community     

Nadia Caidi 《The Information Society》2004,20(1):25-38

Information infrastructures (IIs) are a complex arrangement of people, technology, institutions, content, and conduits. Their development is shaped by the environment in which they evolve and the visions ascribed to them by the various actors. This article examines the assumptions, meanings, and definitions associated with IIs in four Central and Eastern European (CEE) countries: Czech Republic, Hungary, Poland, and Slovakia. In particular, it focuses on how one stakeholder group, the library community, frames the policy debates around IIs. In-depth interviews were conducted in 1999 with 49 library policymakers in 37 institutions. The data shed light on the respondents' collective story and visions and help us gain a better understanding of political and cultural differences in the development of IIs.  相似文献   

中国与欧美电视新闻节目对比研究     

董美静 《沧州师专学报》2011,(2):18-18,125

与欧美国家相比,中国电视的诞生较晚,在北京电视台第一次试播节目时,美国电视业已经用电视媒体与麦卡锡主义进行了英勇搏斗。直至改革开放,中国电视业的发展才真正开始腾飞。在中国电视日渐成熟的今天,放眼欧美电视,分析异同,寻找差距,对中国电视的进一步发展意义很大。以新闻节目为例,对欧美与国内电视节目进行比较。  相似文献   

四川留法勤工俭学生与旅欧党团组织     

田雪梅  鲜于浩 《四川师范大学学报(社会科学版)》2003,30(4):134-140

四川赴法勘工俭学生人数居全国之冠，他们在留法勘工俭学实践中完成了由无政府主义向马克思主义的重大转变，为旅欧党团组织的建立奠定了组织和思想基础；他们还积极参加和领导旅欧党团组织旨在提高党团员政治理论素养的内部训练工作、与无政府主义的斗争以及反对帝国主义和封建军阀的斗争，为中国革命事业的发展立下了不朽功勋。  相似文献   

Researching social inclusion in student mobility: methodological strategies in studying the Erasmus programme     

David Cairns 《International Journal of Research & Method in Education》2019,42(2):137-147

This article explores different methodological approaches to the study of social inclusion in the European Commission’s Erasmus undergraduate exchange programme, elaborating upon the approach taken in a study of Erasmus conducted in Portugal. The opening section of the discussion acknowledges the prior tradition of quantitative research on student mobility in Europe as a means of providing indicators of programmatic success to policymakers and stakeholders, and the existence of micro level studies that examine specific aspects of the Erasmus experience such as the emergence of shared European identities and building work competencies. What these approaches share is an emphasis on student perspectives: learning about the impact of exchange visits from past participants. As an alternative approach, I have focused on the institutional level of the programme, using qualitative interviews with individuals involved in managing Erasmus mobility at a diverse range of universities, an approach that I argue unlocks valuable knowledge about issues such as social inclusion within the programme.  相似文献